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CRD 01-17

Session V: Aquaculture

Stock enhancement: moving from wishful thinking toward sound ecology
Session V: Aquaculture
Abstract No. V-1
ORAL PRESENTATION

Ronald Goldberg, Jose J. Pereira, and Paul Clark
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490

Since the late 1870s, many releases of hatchery-reared juvenile fishes were conducted in Europe and the United States to attempt stock enhancement.  Results of these efforts were largely inconclusive, since the fate of the stocked fish could not be determined adequately.  Beginning in the early 1990s, a renewed interest in stock enhancement emerged, utilizing newly developed fish-marking techniques and a rigorous experimental approach.   Presently, microscopic coded-wire tags provide a way to follow the survival of released fish and biochemical or morphological genetic markers can identify their progeny.  Recent ecological experiments in many different locations and for a number of species have provided statistically valid, quantitative examples of successful stock enhancement.  Adaptive models have been applied, which have systematically identified optimal timing, size at release, stocking density, and release habitats, resulting in cost-effective stock enhancement programs.  Concurrently, codes of practice for responsible enhancement have been established, where risks and benefits are evaluated.  Field studies have been conducted at Milford Laboratory to investigate potential stock enhancement for bay scallops, Argopecten irradians and tautog, Tautoga onitis.  We have characterized nursery habitats for these species and have begun to explore different enhancement strategies.  Our results indicate there is good potential for using aquacultural methods for enhancement when natural recruitment is poor and habitat and environmental conditions are not limiting.

Keywords: stock enhancement, experimental design, bay scallops, tautog


Bay scallop spawning and rearing methods
Session V: Aquaculture
Abstract No. V-2
ORAL PRESENTATION
 

David J. Veilleux
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490

Methods for spawning and rearing of bivalve mollusks established at the Milford Laboratory over the past forty years have led to the development of a commercial aquaculture industry. Currently, the Milford Lab is focusing on aquaculture techniques for the bay scallop, Argopecten irradians irradians. The bay scallop has been selected because of limited wild fishery yield, high market value, and rapid growth.  The methods will be described for the laboratory requirements, conditioning of brood stock, spawning, sampling, larval rearing, feeding, pathogen control and handling.  Our results provide insight on how to achieve batches of post-set scallop spat, ready for any need, such as stock enhancement, or experimental research.  These methods can be extrapolated easily to a commercial scale.


An experimental system for evaluating shellfish recirculating nursery systems
Session V: Aquaculture
Abstract No. V-3
ORAL PRESENTATION

James C. Widman, Jr.
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490

Economical biofiltration of shellfish rearing systems presents unique challenges to the aquaculture industry. Due to these challenges, recirculating systems are used extensively in finfish rearing, but are not used in commercial shellfish culture.  It will be an "open" system with the addition of at least 10% replacement seawater each day.  Each recirculating system will be composed of a biofilter, ultraviolet disinfection, solids removal unit, and protein skimmers to maximize water quality.  Determining which commercially available biofiltration systems are compatible with shellfish culture must be the first step.  Shellfish culture relies on live algal culture for nutritional purposes, however many commercial finfish culture systems are incompatible with live algae.  Process control technology will be used to minimize human intervention (labor costs and biases) throughout the grow-out period. Food consumption will be monitored in order to track food conversion efficiency ratios. Waste production will be monitored and modeled in order to maximize stocking densities of the system while minimizing filtration needs.  To increase stocking densities, we will attempt to minimize periodic ammonia peaks based on the data that are collected.  Management of the system with neural networks or fuzzy logic will be explored once enough empirical data are collected.  Commercial controllers and their conversion for use in the system will be presented.  Data gathered from these experiments will be incorporated into an economic model to determine commercial feasibility.  A brief review of the current state of bay scallop culture in New England will be presented along with the hurdles that need to be overcome to allow a single-season crop.


Rotifer production on microalgal diets: a quantitative approach to developing a feeding strategy
Session V: Aquaculture
Abstract No. V-4
ORAL PRESENTATION

Mark S. Dixon1, Gary H. Wikfors1, and Bethann Balazsi2
1
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490
2Long Island University, Southampton College, Natural Science Division, Southampton, NY 11968

Live food production is a critical component in the successful culturing of finfish. Consistent production of microalgal biomass and the efficient conversion of that biomass into live food lead to reliable nutrition of cultured fish. A series of small-scale experiments designed to optimize microalgal diets, establish a feeding protocol, and define rearing conditions for rotifers (Brachionus plicatilis) was conducted. 

The microalga strain PLY429, Tetraslemis chui, yielded the greatest rotifer production when compared to several commonly-used strains. A constant density of 6 million microalgal cells per milliliter and initial low rotifer stocking densities resulted in rapid reproduction and high overall production. Under these conditions rotifer populations doubled in as little as 2 days, and reached a maximum density of over 2000 per milliliter in 6 days. While higher algal densities produced slightly greater yields, conversion efficiencies were lower.

A spectrophotometer was used to monitor algal densities, algal cells were added manually to maintain the target density, and rotifers were counted manually. There is good potential to automate the entire process using “off the shelf” technology. This potential will be explored in upcoming experiments.


Microalgal production to further science and aquaculture
Session V: Aquaculture
Abstract No. V-5
ORAL PRESENTATION

Barry C. Smith and Gary H. Wikfors
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490

To study organisms, the need to grow them in the laboratory often occurs. This is a type of aquaculture.  In aquaculture a food chain is established as a course of necessity.  If bivalve mollusks are to be grown, all life stages require a microalgal diet.  Many species of finfish feed on rotifers and/or brine shrimp in their early feeding stages. Rotifers and brine shrimp feed on microalgae.  So, to grow species important to aquaculture, their food must also be grown and in adequate supply. Further, the microalgae must also be nutritionally appropriate for the animal to be grown. Other reasons to grow microalgae include the presence of extractable pigments and other chemicals that are abundant in some strains.

To optimize production, aquaculture companies will grow their organisms with several considerations:  House the organism in the most favorable environment possible to minimize physiological stress; Grow them as densely as possible to make best use of space; Feed the organism as much of the proper feeds as needed to get maximum growth. This requires large amounts of microalgal biomass to be grown cost effectively.

The above holds true not only for the animals to be grown but also for the plants. Microalgae need a stable environment within thermal and saline optima. Light of an acceptable wavelength and intensity is required for photosynthesis as well as the proper ratios of nutrients. Contaminants must be excluded or controlled to reduce loss. Once these basic criteria are met, the process can be optimized. To maximize the algal biomass produced, increased nutrients leading to increased density is often preferable to increased volume.

Culture strategy plays a major role in algal production. Batch culture is labor intensive and may not support maximum yield from nutrients. Continuous culture (chemostats and turbidostats) can be unreliable. Semi-continuous culture, so far, is the most economical and reliable strategy, because it combines benefits of continuous culture (opportunity for process control and less labor) and batch culture (better control over modifications to harvest rate in response to changes in culture performance).

Keywords:  aquaculture, algae


An overview of the NOAA diving program 
Session V: Aquaculture
Abstract No. V-6
ORAL PRESENTATION

Barry C. Smith
NOAA/NMFS/NEFSC, 212 Rogers Ave., Milford, CT 06460-6490

Many of the inquiries into the aquatic environment require us to enter the water, not only to observe first hand the organisms and events that occur, but also to accomplish a variety of other tasks. Ships, docks, and other structures need periodic inspection and repair. Monitoring and sampling equipment need installation and regular service. When equipment is lost, it must be recovered to avoid not only monetary loss but also to salvage the scientific information and continue with the given research program. These tasks and more are performed under a wide range of conditions; from crystal clear waters to zero visibility, from tropical seas to ice diving, year-round. Further, the level of complexity ranges from single dives to tending the Aquarius habitat to the “Monitor Project” recovery. The NOAA Diving Program, part of the Office of Marine and Aviation Operations, is the authority that makes sure we enter the water safely and efficiently.

Over 300 divers, 35 Unit Diving Supervisors, and six people staffing the NOAA Diving Center perform over 11,000 dives per year with a safety level of 99.98%.  The majority of the dives are less than 60 feet deep. Other dives can be over 100 feet deep. Most diving uses open circuit SCUBA but several other breathing systems can be employed. The range of tools used is dictated by the task undertaken. Some jobs require sample collection or video documentation while other endeavors may require air chisels or jack hammers.

Keywords: NOAA, diving